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Contents : Chapter 1 : Insectary Operation 1.5 Insectary Manager Responsibilities v 1 Page 1 of 6 1.5 Insectary Manager Responsibilities Mark Benedict To provide authenticated high-quality mosquito reagents training and information to the research community of today and the future in a timely and professional manner. Introduction We begin this section with the mission statement for the MR4 vector activities. In order to accomplish this the following list of responsibilities was developed as guidelines for the MR4 insectary manager. While the details are specific for the MR4 vector activities at the CDC in Atlanta it provides a useful guide for supervisors employing a manager to oversee daily operations and in the development of a job description. With little modification this has served us well to describe the core activities of the manager. Insectary Facilities Environment The Vector Repository Manager (VRM) shall ensure that... 1. Environmental conditions in insectaries are constantly maintained at 27oC ( 0.5 oC). Relative humidity will be controlled to be in the range of 80% ( 10%) 365 days a year without interruption. Lighting is controlled such that a 30 minute sunrise and sunset occur in between which times the fluorescent lights will be on continually. The total darkness between the end of sunset and the beginning of sunrise is 12 hours. 2. The environmental conditions except for lighting are continually monitored by CDC maintenance staff and changes should be made to settings to achieve the above only after consultation and approval by the VRM. 3. Environmental conditions including lighting are continually and independently monitored by the MR4 staff. This is achieved by sensors that are located in all three insectaries and capable of notifying MR4 staff of conditions that are outside of the permissible range within 10 minutes regardless of whether staff are in the MR4 facility at home or traveling as necessary to ensure that at least one staff is aware of the problem. 4. Pest insect control is continually performed to ensure essential absence of ants and cockroaches. This is achieved in a way that no harm occurs to the insect colonies either directly or by contamination with toxicants transported by pests. In the event that other pests are observed (e.g. mice) control is enacted as needed but again with highest regard for the health of the repository insects. Modifications of the facility are considered that physically reduce entry points breeding sites and harborages. 5. Insect pest control around the perimeter of the building to reduce external sources is considered and exercised if needed. 6. Neither CDC personnel nor local municipalities conduct insect control in the vicinity of the insectary facilities. 7. Properly operating mosquito traps or other killing devices operate continuously and are monitored for catches in all insectaries. These should be capable of trapping primarily Anopheles but also Aedes and Culex species. 8. Cleanliness is maintained in all insectaries and support areas. While hospital cleanliness is neither attainable nor necessary a consistent effort should be made to improve the level of cleanliness. In part this will require labor but use of materials and furnishings that do not rust and are easily cleaned will be helpful. Only cleaning compounds that are non-toxic to the mosquitoes are used but these should be used to reduce cleaning maintenance when possible. Moreover the CDC maintenance Chapter 1 : Insectary Operation 1.5 Insectary Manager Responsibilities v 1 Page 2 of 6 staff is instructed to maintain the cleanliness of the floor and other areas within their responsibility. The VRM is responsible for cleanliness but is not to become the custodian. 9. Air filtration is installed and maintained properly to reduce the level of odors fungi dust hair etc. Installing additional equipment or modifying existing equipment is considered to improve the air quality. Mold growing on mosquitoes and the insectary walls can be reduced by consistent attention to eliminating spores. Centralized UV sterilization of the air may be feasible. 10. Ensure that documentation of the maintenance of the emergency generator is available and being maintained. Notify the PI in the event of any planned power outage. Infrastructure Improvements The VRM shall ensure that... 1. Sign-holders are installed that contain information about specific courses of action to take in the event of various environmental anomalies. These will be located either near the alarms and/or by each doorway. 2. Signage is current and attractively maintained. 3. All infrastructure and environmental changes are consistent with the MR4 objectives. Furthermore these changes are approved by all insectary users. Infrastructure Maintenance The VRM shall ensure that... 1. Hallways are kept clear of trash boxes unused carts old equipment etc. 2. All lights function. The maintenance personnel should be n

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