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Contents : Physiological Science 111L Laboratory Manual Winter Quarter 2010 Physiological Science 111L Laboratory Manual Preface This course is designed to provide hands on experience in experimental aspects of data acquisition and analysis techniques and together with the use of computer simulations will illustrate important physiological principles. In setting up this course the approach has been to utilize wherever possible state-of-the-art instrumentation and analysis techniques and thus to provide exposure to the technological environment likely to be encountered in clinical and research laboratories in the future. Experimental data will be collected and analyzed using a Power Macintosh computer interfaced with a MacLab data acquisition system and the first laboratory session is designed to provide familiarity with the use of this instrumentation. The nine subsequent laboratory modules will each address a significant physiological topic including the nerve action potential examined experimentally and by computer simulation neuromuscular function skeletal muscle mechanics measurement of EKG and respiratory parameters epithelial transport and reproductive physiology. The course content complements the lecture material in the Foundations of Physiology 111 course series (PS 111A B C) Contents Introduction Course Outline Guidelines concerning use of animals Laboratory safety Participation in exercises to measure human physiological responses Personnel involved in 111L Instructional Policy and Evaluation of Performance Use of the MacLab data acquisition and analysis system Computer Simulation of Action Potential Electromyography Human Electrocardiogram and Blood Pressure Compound Action Potential Frog Sciatic Nerve Frog Skeletal Muscle Contraction Human Respiration Measurement of Active Sodium Transport in Frog Skin Computer Simulation of Water and Solute Movement in the Kidney Hormonal Control of Reproduction Evaluation of Estrus Cycling in the Rat Page i ii iii iii iv v viii 2.1 3.1 4.1 5.1 6.1 7.1 8.1 9.1 10.1 Introduction I. Course Outline: Week 1 2 Laboratory Introduction Course overview and use of the MacLab data acquisition system Computer Simulation of Action Potential the effect of varying the extracellular and intracellular ionic environment on the resultant action potential will facilitate understanding of the role of various ion channels in this response Electromyography measurement of electrical signals by surface electrodes placed on the biceps brachii will provide insight into neuromuscular function. Human Electrocardiogram and Blood Pressure the technique for obtaining an electrocardiogram (EKG) will be performed along with measurement of arterial blood pressure Compound Action Potential Extracellular recording from isolated frog sciatic nerve will illustrate the concept of threshold for action potential initiation and the contribution of different fiber types to the overall response. Muscle Physiology relationships between muscle length force and velocity of contraction will be studied using isolated frog skeletal muscle. Human Respiration measurements of tidal volume expiratory reserve volume inspiratory reserve volume and vital capacity will be performed. Membrane transport in epithelia measurement of active sodium transport in frog skin will demonstrate principles of electrogenic transport Computer Simulation of Water and Solute Movement in the Kidney simulation programs will focus on glomerular capillary dynamics proximal tubular reabsorption and the distal nephron Reproductive Physiology stages of the estrus cycle will be examined by cytological staining and correlated with behavioral changes 3 4 5 6 7 8 9 10 2. Guidelines concerning use of animals: The Animal Care and Use training Manual published by the Chancellor's Animal Research Committee provides guidelines on the care and use of laboratory animals at UCLA (a copy is available in the laboratory). As stated in the preface to the manual UCLA recognizes that the use of animals for teaching and research is fundamental to biology and medicine. In setting up the present course a great deal of effort has been taken to design experiments which demonstrate important physiological principles and at the same time minimizes the use of animals. All experiments involving the use of animals (frogs and rats) have been approved by the Chancellor's Animal Research Committee. Since you will be working with live rats in week 10 you will be required to (i) observe a video dealing with procedures for correct handling of rats and (ii) complete a UCLA Medical History Questionnaire (Health Questionnaire) ONLINE. These two items will be addressed in week 7.. 3. Laboratory Safety Please no eating or drinking in the laboratory. Take extreme care when using any sharp instrument during dissections. Disposable latex gloves are provided for your use when performing dissections. For human performance measurements disposable supplies will be used whenever feasible spirometers will be sterilized before use. In performing wet lab experiments (weeks 5.6 and 8) take care to avoid contact of solutions with the computer equipment keep all tissue preparation materials to the right hand area of the bench as far removed as possible from the computers. 4. Participation in exercises to measure human physiological responses: Several of the laboratory sessions will involve making simple non-invasive measurements of various physiological responses e.g. blood pressure EKG and respiratory measurements on other student volunteers and examining how these responses may be influenced by the state of physical activity of the person eg. when using an exercise bicycle. If you have any medical condition that may be adversely affected by participating in these exercises you should not volunteer to perform these exercise routines. 5. PS111L Website As with other undergraduate classes there will be a website devoted to this class. The class schedule is listed there and it contains a bulletin board for your use. You should periodically check the site for any updated information about the course. Personnel involved in 111L Instructor: Dr. Ronald H. Cooper Office: 1812 Life Science Building Phone: 206 3201 Email: rcooper@physci.ucla.edu OH: Tues 10 11 am Fri 9.30 10.30 am

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